Storyline Slide Target Won't Work in Preview

On certain Storyline 2 slides, where I have layers, I can't get the layers to play in the preview. On other slides, the preview works just fine.

Note: I have one slide with 51 layers. I copied that slide and used it to create additional slides.  I simply edited certain layers and the deleted the rest of the layers I didn't need from the original slide.  It has previewed for some slides. It doesn't preview however on my slides where I only have 1-3 layers.  

Is there something I need to click or unclick?

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