Hello Rachna.
Indeed, there is a small bug on your hotspot slide.
I can see it. I (and you) can fix it. But I can't explain it.
If you look closely at your slide with the incorrect layer, you can see on the base layer that the rotating selection marker is visible on your submit button (it shouldn't).
This means that we press the Submit button AND choose that (incorrect) area at the same time, every time, even though we clicked red first.
In other words, when you click the Submit button, you are simultaneously clicking an incorrect area.
The proof: if you use the submit button of the player (after having clicked on the red one), the correct layer appears, of course.
Look again: if you remove the freeform from your slide and then put it back, you need to reassign the click zone (unassigned at this time) in the submit button trigger. Do not go too fast... When you hover over the single click zone offered in the dropdown list, you can see on the base layer that the area circled in red almost completely covers the layer, including your button. This causes a conflict: submit button = wrong click box, so the wrong layer appears. IMHO, this comes from the software.
I have tried a lot but failed to resolve this issue. Maybe someone else.
The only solution I found is this: you redo your slide from scratch by copying and pasting all of your elements to a freeform hotspot slide EXCEPT your submit button. You create a new submit button in the same place. In triggers, you set this button to validate this interaction. And yes ! the click zone circled in red on the layer "seems" to cover your button but it now works.
Be careful, if you remove the freeform and then reinstall it, the bug returns.
Hope I helped and made it clear enough.