Audio on a single layer Plays in Preview but not when Published as Video

I have created a series of micro-videos using Storyline 360. In my final draft of one of the videos one of the layers (3rd layer, Bob sitting at desk) the audio track is not playing in the video. It plays fine in the preview and worked in previous draft versions. I have tried to delete and recreate the layer, I have created a copy of the entire Storyline file, and am at a loss as to how to fix this. I am really hoping someone can help!!! I have attached the Storyline file and the video file.

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