Does it matter what layer an audio file is on if you don't want it to play when someone revisits the slide?

Thanks for sharing, but I don't see any triggers set for slide 1 except for the one submit the answer. I know that I am missing triggers, I just don't know how to phrase them so that the trigger works for the question on the base layer, (I think I got that one) with one for the review layer. I either can't get it to play at all, or it always does on revisiting a slide from the menu, or the previous button.